Strategies for adaptation of mAb-producing CHO cells to serum-free medium
نویسندگان
چکیده
منابع مشابه
Strategies for adaptation of mAb-producing CHO cells to serum-free medium
Background The large-scale production of biopharmaceuticals, such as monoclonal antibodies (mAbs), commonly requires the use of serum-free medium, for both safety and economical reasons. However, because serum is such an essential supplement of the growth medium of most mammalian cells, its removal demands a very time-consuming process of cell adaptation. In this process, cells are usually subj...
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Background Deficiency of the lysosomal glucocerebrosidase (GCR) enzyme results in Gaucher disease, the most common inherited storage disorder [1]. Current treatment consists of enzyme replacement therapy by administration of exogenous GCR. Although effective, it is exceptionally expensive [2]. In Brazil, the public healthcare system provides the drug free of charge for all Gaucher’s patients, w...
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Introduction Mammalian cell culture medium development has widely evolved in recent years. The use of hydrolysates as serum replacement has led to process variability due to lot-tolot variations. The undefined composition of these media could also increase the process optimization timelines, sometimes with limited impact on process performances. With the reduction of process development activit...
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Currently, mammalian cell technology has become the focus of biopharmaceutical production, with strict regulatory scrutiny of the techniques employed. Major concerns about the presence of animal-derived components in the culture media led to the development of serum-free (SF) culture processes. However, cell adaptation to SF conditions is still a major challenge and limiting step of process dev...
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To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have focused on two transfection vehicles: polyethylenimine (PEI) and calcium phosphate (CaPi). Both have been shown to result in high transfection efficiencies at scales beyond 10 L. Unfortunately, both approaches yield higher levels of recombinant protein (r-protein) in the presence of serum than in...
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ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2011
ISSN: 1753-6561
DOI: 10.1186/1753-6561-5-s8-p112